Press "Enter" to skip to content

How to use microscope, steps and precautions

admin 0

The optical microscope is composed of eyepiece, objective lens, coarse quasi focus screw, fine quasi focus screw, tablet holder, through hole, light shield, converter, reflector, stage, mirror arm, lens barrel, lens base, concentrator and diaphragm. The following is a small arrangement of the use of the microscope, I hope to help you.

How to use the microscope

1. When taking and placing the microscope, first take out the microscope from the mirror box. Hold the mirror arm with one hand and hold the mirror base with the other hand. Keep the mirror body upright. Do not use one hand to tilt and carry it to prevent dropping the eyepiece. Take and put it gently, with the mirror arm facing towards you, 5-10 cm away from the edge of the table. The table should be balanced, clean and free from direct sunlight.

2. Turn on the light source and turn on the power switch.

3. Place the slide specimen. Place the slide specimen to be examined on the stage so that the material is facing the center of the through hole. Then the slide was clamped on both ends of the slide by spring to prevent the slide specimen from moving. If it is a slide mover, slide the slide specimen into the slide shifter, then adjust the glass shifter to move the material to the center of the hole.

4. When observing specimen with microscope, the object image should be found with low power objective first. Because of the large observation range of the low power objective, it is easy to find the object image and the parts that need to be carefully observed.

The method is as follows: (1) rotate the coarse adjustment screw, and observe from the side with eyes, so that the lens barrel drops until the low power objective is about 0.5cm away from the specimen.

(2) Use the left eye to observe from the eyepiece, open the right eye naturally, and slowly turn the coarse adjustment screw by hand to make the lens tube rise gradually until the object image in the field of vision is clear. After that, the fine-tuning screw was used to adjust the focal length slightly to make the object image the clearest.

(3) Move the slide back and forth or adjust the slide shifter to find the part to be observed. It should be noted that the object image in the field of vision is inverted, and the slide should be moved in the opposite direction.

Precautions for use of microscope

The microscope must be handled with care. Hold the mirror arm with your right hand and hold the mirror base with your left hand. When placing, let the front end of the mirror base contact the desktop first, and then gently put down the whole mirror base to avoid the mirror body from vibration. In order to prevent the lens from being polluted, the following measures should be taken:

1. Don’t touch the lens with your fingers to avoid perspiration;

2. When lowering the lens barrel, be sure to look at the objective lens from the side to prevent the objective lens from touching the cover glass slide and damaging the glass slide specimen and objective lens;

3. When observing fresh specimens, be sure to cover the slide and absorb the excess water or solution on the slide;

4. After each use of the microscope, the eyepiece and objective lens should be cleaned with lens cleaning paper. Don’t rotate the collimation screw at will. When observing, you must first adjust the focal length with the coarse collimation screw, and then fine tune with the fine collimation screw after seeing the object image clearly. Because there is a certain range of fine quasi focus screw rotation, when the rotation does not move, the coarse quasi focus screw should be rotated in the opposite direction, and then the fine quasi focus screw should be used to adjust. Do not rotate hard to avoid damaging the gear. After the observation, when the slide is removed from the stage, the lens barrel must be raised to avoid the slide hitting the objective. Then turn the converter and turn the objective lens to the front to form an “eight” shape.

The purpose of a microscope is to magnify images of tiny objects. It is generally used in the observation of biology, medicine and microparticles.

(1) Use the movement of the slightly moving stage, with full eyepiece of the cross mark, for length measurement.

(2) Use the differential angle dial of the rotary stage and the lower end of the eyepiece, with the address base marking of the fully closed eyepiece, to measure the angle, so that one end of the angle to be measured is aligned with the crosshair and coincides with it, and then the other end is also coincided.

(3) The pitch, pitch diameter, outside diameter, tooth angle and tooth shape of the thread are detected by standard.

(4) Check the grain condition of metallographic surface.

(5) Inspect the machined surface of the workpiece.

Leave a Reply

Your email address will not be published. Required fields are marked *